Stat3 Knockout cell line (Raw 264.7)

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Stat3 Knockout cell line (Raw 264.7) Order

Stat3 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0041
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Stat3 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Stat3 Knockout cell line (Raw 264.7)
Species
Gene Name
Stat3
Gene ID
20848
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Stat3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Stat3 gene knockout Stat3 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Stat3 Knockout cell line (Raw 264.7)
Stat3 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0041
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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